Hotspot

ycf1

IRb · 109300-110311 · 1012 bp

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Pi 0.00101

MarkerSeek score 46.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1012
variable_sites: 1
pi: 0.00101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCCTTTGGTGTCATTGACA	ACTGGGGCGATAAGAGGATT	50.0	50.0	58.0	58.2	1404-1450 bp	1.0	81.8
ycf1_p2	CCCGGAACTTCTTCGTAGTG	ACTGGGGCGATAAGAGGATT	55.0	50.0	58.0	58.2	1513-1559 bp	1.0	81.6
ycf1_p3	CCGGAACTTCTTCGTAGTGG	ACTGGGGCGATAAGAGGATT	55.0	50.0	58.0	58.2	1512-1558 bp	1.0	81.6
ycf1_p4	GAGCTACTGAGGAACAACGG	ACTGGGGCGATAAGAGGATT	55.0	50.0	58.0	58.2	1608-1654 bp	1.0	81.4
ycf1_p5	GAGCTACTGAGGAACAACGG	ACAACATCCTTTACTGGGGC	55.0	50.0	58.0	57.8	1620-1666 bp	1.0	81.2