Hotspot

rbcL

LSC · 56251-57684 · 1434 bp

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Pi 0.00767

MarkerSeek score 63.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 11
pi: 0.00767
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CGCCTAATTCATTCGTGTCG	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.4	58.4	1564 bp	1.0	72.5
rbcL_p2	CGCCTAATTCATTCGTGTCG	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.4	58.4	1565 bp	1.0	72.5
rbcL_p3	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.1	58.4	1513 bp	1.0	70.0
rbcL_p4	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.1	58.4	1514 bp	1.0	70.0
rbcL_p5	TTCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	47.6	45.0	57.8	58.4	1514 bp	1.0	68.0