Hotspot

ndhD

SSC · 115616-117118 · 1503 bp

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Pi 0.00266

MarkerSeek score 47.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 4
pi: 0.00266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTCAGCAATTGTCGCGTCTA	GATACCCTGGGACGGATGTA	45.0	55.0	57.9	58.0	2154-2157 bp	1.0	80.4
ndhD_p2	GCAATTGTCGCGTCTATTGG	GATACCCTGGGACGGATGTA	50.0	55.0	57.9	58.0	2150-2153 bp	1.0	80.3
ndhD_p3	GAAGCGTGGGGATCCTATTG	GATACCCTGGGACGGATGTA	55.0	55.0	58.1	58.0	2270-2273 bp	1.0	80.0
ndhD_p4	TTTGTGTGAACTGGCGAGAA	GATACCCTGGGACGGATGTA	45.0	55.0	58.0	58.0	1977 bp	0.5	59.7
ndhD_p5	TTTGTGTGAACTGGCGAGAA	ACAAATCGCTTCTGCTCCAA	45.0	45.0	58.0	58.1	1950 bp	0.5	58.9