Hotspot

rps15-ycf1

SSC · 123459-123825 · 367 bp

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Pi 0.0227

MarkerSeek score 57.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 367
variable_sites: 8
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCGAACTGAATGAATTTTTGACC	ACGATTGAAAATTCGTGGAT	39.1	35.0	56.9	53.6	400-414 bp	1.0	44.3
rps15-ycf1_p2	AGATCCTCTGTTTTCTTCGT	ACGATTGAAAATTCGTGGAT	40.0	35.0	54.0	53.6	438-452 bp	1.0	44.0
rps15-ycf1_p3	CAGATCCTCTGTTTTCTTCGT	ACGATTGAAAATTCGTGGAT	42.9	35.0	55.6	53.6	439-453 bp	1.0	43.9
rps15-ycf1_p4	CAGATCCTCTGTTTTCTTCGTT	ACGATTGAAAATTCGTGGAT	40.9	35.0	56.4	53.6	439-453 bp	1.0	43.9
rps15-ycf1_p5	CAGATCCTCTGTTTTCTTCGTTT	ACGATTGAAAATTCGTGGAT	39.1	35.0	57.1	53.6	439-453 bp	1.0	43.9