Hotspot

ccsA-ndhD

SSC · 115364-115615 · 252 bp

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Pi 0.0286
MarkerSeek score 71.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
252
variable_sites
7
pi
0.0286
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TTCAGCAATTGTCGCGTCTA ACCCGTACTAGGTATTGGTATGT 45.0 43.5 57.9 58.2 458-461 bp 1.0 56.3
ccsA-ndhD_p2 GCAATTGTCGCGTCTATTGG ACCCGTACTAGGTATTGGTATGT 50.0 43.5 57.9 58.2 454-457 bp 1.0 56.2
ccsA-ndhD_p3 AATTCAGCAATTGTCGCGTC ACCCGTACTAGGTATTGGTATGT 45.0 43.5 57.8 58.2 460-463 bp 1.0 54.9
ccsA-ndhD_p4 TCAGCAATTGTCGCGTCTAT ACCCGTACTAGGTATTGGTATGT 45.0 43.5 57.7 58.2 457-460 bp 1.0 54.6
ccsA-ndhD_p5 TTCAGCAATTGTCGCGTCTA CCCGTACTAGGTATTGGTATGT 45.0 45.5 57.9 56.5 457-460 bp 1.0 54.0