Hotspot

ndhF-rpl32

SSC · 112638-113154 · 517 bp

Back to Sesamum

Pi 0.0252

MarkerSeek score 69.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 517
variable_sites: 13
pi: 0.0252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACGAAAGGTATGATCCACGC	GCCCAATACCCCTTCCTTTT	50.0	50.0	58.1	57.8	636-638 bp	1.0	79.4
ndhF-rpl32_p2	CGAAAGGTATGATCCACGCA	GCCCAATACCCCTTCCTTTT	50.0	50.0	58.1	57.8	635-637 bp	1.0	79.4
ndhF-rpl32_p3	CTGGAAGTGGAACGAAAGGT	GCCCAATACCCCTTCCTTTT	50.0	50.0	57.7	57.8	647-649 bp	1.0	77.8
ndhF-rpl32_p4	ACGAAAGGTATGATCCACGC	AAAACGCTTTTAACGCTGCC	50.0	45.0	58.1	58.5	653-655 bp	1.0	77.2
ndhF-rpl32_p5	CGAAAGGTATGATCCACGCA	AAAACGCTTTTAACGCTGCC	50.0	45.0	58.1	58.5	652-654 bp	1.0	77.2