Hotspot

ycf1-ndhF

SSC · 110312-110381 · 70 bp

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Pi 0.267

MarkerSeek score 74.70

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 70
variable_sites: 12
pi: 0.267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GGCGGATCCAAACAAAATCG	CTTTTTGATCTTACTTTTGAATCA	50.0	25.0	58.1	52.3	188-213 bp	1.0	48.8
ycf1-ndhF_p2	GGCGGATCCAAACAAAATCG	ACTTTTTGATCTTACTTTTGAATC	50.0	25.0	58.1	52.3	189-214 bp	1.0	48.8
ycf1-ndhF_p3	GAAGGAAAGGCGGATCCAAA	CTTTTTGATCTTACTTTTGAATCA	50.0	25.0	58.2	52.3	196-221 bp	1.0	48.6
ycf1-ndhF_p4	GAAGGAAAGGCGGATCCAAA	ACTTTTTGATCTTACTTTTGAATC	50.0	25.0	58.2	52.3	197-222 bp	1.0	48.6
ycf1-ndhF_p5	GGCGGATCCAAACAAAATCG	TCTATTTCTCTTGTGTATCAATTT	50.0	25.0	58.1	52.2	235-260 bp	1.0	47.9