Hotspot

ycf3-tRNA-Ser (GGA)

LSC · 45008-45871 · 864 bp

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Pi 0.0162

MarkerSeek score 63.30

Primer available yes

Label ycf3-tRNA-Ser (GGA)

Feature Metadata

label_name: ycf3-tRNA-Ser (GGA)
feature_type: igs
region: LSC
length_bp: 864
variable_sites: 14
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-tRNA-Ser (GGA)_p1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	1036-1061 bp	1.0	73.4
ycf3-tRNA-Ser (GGA)_p2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	1035-1060 bp	1.0	73.4
ycf3-tRNA-Ser (GGA)_p3	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	1057-1082 bp	1.0	72.9
ycf3-tRNA-Ser (GGA)_p4	GCCTCCTTTTCTCCTGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	1056-1081 bp	1.0	72.9
ycf3-tRNA-Ser (GGA)_p5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.1	57.8	1007-1032 bp	1.0	72.6