Hotspot

tRNA-Lys (UUU)-rps16

LSC · 4247-5135 · 889 bp

Back to Sesamum

Pi 0.0147
MarkerSeek score 60.00
Primer available yes
Label tRNA-Lys (UUU)-rps16

Feature Metadata

label_name
tRNA-Lys (UUU)-rps16
feature_type
igs
region
LSC
length_bp
889
variable_sites
13
pi
0.0147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Lys (UUU)-rps16_p1 AAAGCCGAGTACTCTACCGT GGAACTTCCGTCTTCCGTC 50.0 57.9 58.2 57.9 944-945 bp 1.0 71.0
tRNA-Lys (UUU)-rps16_p2 AAGCCGAGTACTCTACCGTT GGAACTTCCGTCTTCCGTC 50.0 57.9 58.2 57.9 943-944 bp 1.0 71.0
tRNA-Lys (UUU)-rps16_p3 TCTAGCCGCACTTAAAAGCC GGAACTTCCGTCTTCCGTC 50.0 57.9 58.3 57.9 958-959 bp 1.0 70.2
tRNA-Lys (UUU)-rps16_p4 CCGCACTTAAAAGCCGAGTA GGAACTTCCGTCTTCCGTC 50.0 57.9 58.3 57.9 953-954 bp 1.0 70.0
tRNA-Lys (UUU)-rps16_p5 AAGCCGAGTACTCTACCGTT AGGAACTTCCGTCTTCCGT 50.0 52.6 58.2 58.3 944-945 bp 1.0 69.9