Hotspot

tRNA-His (GUG)-psbA

LSC · 91-382 · 292 bp

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Pi 0.0205
MarkerSeek score 64.30
Primer available yes
Label tRNA-His (GUG)-psbA

Feature Metadata

label_name
tRNA-His (GUG)-psbA
feature_type
igs
region
LSC
length_bp
292
variable_sites
6
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-His (GUG)-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 359-363 bp 1.0 79.7
tRNA-His (GUG)-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 373-377 bp 1.0 79.6
tRNA-His (GUG)-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 372-376 bp 1.0 79.6
tRNA-His (GUG)-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 374-378 bp 1.0 79.6
tRNA-His (GUG)-psbA_p5 GATCCACTTGGCTACATCCG CAACCGTGCTAACCTTGGTA 55.0 50.0 58.1 57.8 411-415 bp 1.0 78.7