Pi
0.0395
MarkerSeek score
63.10
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | GAGCTACTGAGGAACAACGG | GTAGGGGGTGGTCGAATTTC | 55.0 | 55.0 | 58.0 | 58.0 | 988-1846 bp | 1.0 | 89.7 |
| ycf1_p2 | GAGCTACTGAGGAACAACGG | TAGGGGGTGGTCGAATTTCT | 55.0 | 50.0 | 58.0 | 58.0 | 987-1845 bp | 1.0 | 89.7 |
| ycf1_p3 | GAGCTACTGAGGAACAACGG | GAATTCCAAATGGGGTGGGT | 55.0 | 50.0 | 58.0 | 58.0 | 1044-1902 bp | 1.0 | 89.5 |
| ycf1_p4 | GCAAAAATGCCTTCCTCGTC | GTAGGGGGTGGTCGAATTTC | 50.0 | 55.0 | 58.0 | 58.0 | 1378-2236 bp | 1.0 | 88.6 |
| ycf1_p5 | GAGCTACTGAGGAACAACGG | TGGAATTCCAAATGGGGTGG | 55.0 | 50.0 | 58.0 | 58.1 | 1277-1904 bp | 0.461538 | 61.5 |
| ycf1_p1 | TCTGTGCCAAGGTTTCAGAC | ATCCCTTTTTGAACGGACCC | 50.0 | 50.0 | 58.0 | 58.1 | 1187-1199 bp | 1.0 | 88.3 |
| ycf1_p2 | TGCCAAGGTTTCAGACGAAA | ATCCCTTTTTGAACGGACCC | 45.0 | 50.0 | 58.0 | 58.1 | 1183-1195 bp | 1.0 | 88.2 |
| ycf1_p3 | ATCCTCTGACCATTCGGGAA | ATCCCTTTTTGAACGGACCC | 50.0 | 50.0 | 58.1 | 58.1 | 1043-1055 bp | 1.0 | 87.8 |
| ycf1_p4 | TCCTCTGACCATTCGGGAAT | ATCCCTTTTTGAACGGACCC | 50.0 | 50.0 | 58.1 | 58.1 | 1042-1054 bp | 1.0 | 87.8 |
| ycf1_p5 | CCGAAAATCCGGTTGTTGTG | ATCCCTTTTTGAACGGACCC | 50.0 | 50.0 | 58.0 | 58.1 | 614-632 bp | 0.769231 | 80.1 |