Hotspot

ndhF

SSC · 114907-117159 · 2253 bp

Pi 0.0244

MarkerSeek score 57.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 163
pi: 0.0244
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCACCTAGCATTGGGTAGAC	AACTGAACCCTCTCAAAAGA	55.0	40.0	58.0	54.1	2634-2968 bp	0.769231	48.9
ndhF_p2	GCTCGGGTTATGGAAGAAGG	AACTGAACCCTCTCAAAAGA	55.0	40.0	58.0	54.1	2735-2979 bp	0.615385	41.8
ndhF_p3	TCTCTTCCTTCTCCGAGCTC	AACTGAACCCTCTCAAAAGA	55.0	40.0	58.2	54.1	2751-2995 bp	0.615385	40.2
ndhF_p4	AGGGCCCTCTTATCTCTTCC	AACTGAACCCTCTCAAAAGA	55.0	40.0	57.9	54.1	2763-2997 bp	0.384615	31.2
ndhF_p5	GGGCCCTCTTATCTCTTCCT	AACTGAACCCTCTCAAAAGA	55.0	40.0	57.9	54.1	2762-2996 bp	0.384615	31.2