Hotspot

ndhG-ndhI

SSC · 123246-123680 · 435 bp

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Pi 0.101
MarkerSeek score 70.30
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
435
variable_sites
111
pi
0.101
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.92
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 ATCGGTTCAGGTAAATCCAT TGACGATTATACAATTCGAACA 40.0 31.8 54.1 53.7 430-593 bp 1.0 52.0
ndhG-ndhI_p2 ATCGGTTCAGGTAAATCCAT TGACGATTATACAATTCGAACAA 40.0 30.4 54.1 54.5 430-593 bp 1.0 52.0
ndhG-ndhI_p3 ATCGGTTCAGGTAAATCCAT TTGACGATTATACAATTCGAACA 40.0 30.4 54.1 54.5 431-594 bp 1.0 52.0
ndhG-ndhI_p4 ATCGGTTCAGGTAAATCCAT TTGACGATTATACAATTCGAACAA 40.0 29.2 54.1 55.3 431-594 bp 1.0 52.0
ndhG-ndhI_p5 ATCGGTTCAGGTAAATCCAT ACAATTCGAACAATTCAATTCA 40.0 27.3 54.1 53.2 420-583 bp 1.0 52.0