Hotspot

rps3-rps19

LSC · 87912-88594 · 683 bp

Back to Senna

Pi 0.0652
MarkerSeek score 78.10
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
683
variable_sites
128
pi
0.0652
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.95
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 TACCAAGTCTGAAACCGAGC AAATGATAATAGATCTCGTCGT 50.0 31.8 57.5 52.7 691-929 bp 1.0 52.0
rps3-rps19_p2 TACCAAGTCTGAAACCGAGC AATGATAATAGATCTCGTCGTT 50.0 31.8 57.5 52.7 690-928 bp 1.0 52.0
rps3-rps19_p3 GTCTGAAACCGAGCGGATTT AAATGATAATAGATCTCGTCGT 50.0 31.8 58.6 52.7 685-923 bp 1.0 52.0
rps3-rps19_p4 GTCTGAAACCGAGCGGATTT AATGATAATAGATCTCGTCGTT 50.0 31.8 58.6 52.7 684-922 bp 1.0 52.0
rps3-rps19_p5 TACCAAGTCTGAAACCGAGC AAATGATAATAGATCTCGTCGTT 50.0 30.4 57.5 53.5 691-929 bp 1.0 52.0