Hotspot

trnG-GCC-trnM-CAU

LSC · 40500-40669 · 170 bp

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Pi 0.0517
MarkerSeek score 73.30
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
170
variable_sites
27
pi
0.0517
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 CTTTGCCAAAGAGAAGACGC CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.7 58.0 247-267 bp 1.0 86.7
trnG-GCC-trnM-CAU_p2 CTTTGCCAAAGAGAAGACGC TGAGGTCACGGGTTCAAATC 50.0 50.0 57.7 57.8 245-265 bp 1.0 85.5
trnG-GCC-trnM-CAU_p3 CTTTGCCAAAGAGAAGACGC TAGCTCGCAAGGCTCATAAC 50.0 50.0 57.7 57.8 267-287 bp 1.0 84.8
trnG-GCC-trnM-CAU_p4 CTTTGCCAAAGAGAAGACGC GTAGCTCGCAAGGCTCATAA 50.0 50.0 57.7 57.8 268-288 bp 1.0 84.7
trnG-GCC-trnM-CAU_p5 CTTTGCCAAAGAGAAGACGC GAGGTCACGGGTTCAAATCC 50.0 55.0 57.7 58.5 244-264 bp 1.0 82.6