Hotspot

rps16-trnQ-UUG

LSC · 6629-7283 · 655 bp

Back to Senna

Pi 0.0899
MarkerSeek score 77.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
655
variable_sites
152
pi
0.0899
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 588-1090 bp 1.0 89.8
rps16-trnQ-UUG_p2 AGTCGCACGTTGCTTTTTAC GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 592-1094 bp 1.0 88.4
rps16-trnQ-UUG_p3 CAAGTCGCACGTTGCTTTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.5 58.1 594-1096 bp 1.0 87.7
rps16-trnQ-UUG_p4 GTCGCACGTTGCTTTTTACC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.6 58.1 591-1093 bp 1.0 86.5
rps16-trnQ-UUG_p5 GCACGTTGCTTTTTACCACA TTCGGAGGTTCGAATCCTTC 45.0 50.0 57.8 57.3 592-1094 bp 1.0 85.1