Hotspot

rps3-rps19

LSC · 91264-92050 · 787 bp

Back to Senegalia

Pi 0.0757

MarkerSeek score 81.10

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 70
pi: 0.0757
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	GTCTGAAACCGAGCGGATTT	AAATGATAATAGATCTCGTCGT	50.0	31.8	58.6	52.7	676-846 bp	1.0	51.2
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	AATGATAATAGATCTCGTCGTT	50.0	31.8	58.6	52.7	675-845 bp	1.0	51.2
rps3-rps19_p3	TACCAAGTCTGAAACCGAGC	AAATGATAATAGATCTCGTCGT	50.0	31.8	57.5	52.7	682-852 bp	1.0	51.1
rps3-rps19_p4	TACCAAGTCTGAAACCGAGC	AATGATAATAGATCTCGTCGTT	50.0	31.8	57.5	52.7	681-851 bp	1.0	51.1
rps3-rps19_p5	TACCAAGTCTGAAACCGAGC	AAATGATAATAGATCTCGTCGTT	50.0	30.4	57.5	53.5	682-852 bp	1.0	51.1