Hotspot

rpl33-rps18

LSC · 74608-74984 · 377 bp

Back to Senegalia

Pi 0.0457

MarkerSeek score 78.30

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 22
pi: 0.0457
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	481-513 bp	1.0	84.4
rpl33-rps18_p2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	482-514 bp	1.0	84.4
rpl33-rps18_p3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.6	57.7	483-515 bp	1.0	82.6
rpl33-rps18_p4	TCTGTCCCTGTTGTTGCAAA	GGGCAAACGTCTACGAAAAG	45.0	50.0	57.9	57.4	455-487 bp	1.0	81.9
rpl33-rps18_p5	TTCTGTCCCTGTTGTTGCAA	GGGCAAACGTCTACGAAAAG	45.0	50.0	57.9	57.4	456-488 bp	1.0	81.9