Hotspot

ycf4-cemA

LSC · 66612-67045 · 434 bp

Back to Senegalia

Pi 0.0236

MarkerSeek score 73.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 15
pi: 0.0236
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AGATCCACCAGGGCAAAAAG	45.0	50.0	58.1	58.1	543-562 bp	1.0	83.2
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.1	58.1	544-563 bp	1.0	83.1
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GATCCACCAGGGCAAAAAGA	45.0	50.0	58.1	58.1	542-561 bp	1.0	83.1
ycf4-cemA_p4	ACAAAAAGCGGCAGAATTGG	AGATCCACCAGGGCAAAAAG	45.0	50.0	57.8	58.1	559-578 bp	1.0	82.6
ycf4-cemA_p5	ACAAAAAGCGGCAGAATTGG	GATCCACCAGGGCAAAAAGA	45.0	50.0	57.8	58.1	558-577 bp	1.0	82.5