Hotspot

ndhC-trnM-CAU

LSC · 56447-58245 · 1799 bp

Back to Senegalia

Pi 0.0326
MarkerSeek score 67.90
Primer available yes
Label ndhC-trnM-CAU

Feature Metadata

label_name
ndhC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1799
variable_sites
72
pi
0.0326
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAU_p1 TCATATTCGTAAAGCAAAAACA CCCGCCGTATGAAAGCAATA 27.3 50.0 52.7 58.4 1404-1963 bp 1.0 47.3
ndhC-trnM-CAU_p2 TCATATTCGTAAAGCAAAAACA CCGCCGTATGAAAGCAATAC 27.3 50.0 52.7 57.3 1403-1962 bp 1.0 47.3
ndhC-trnM-CAU_p3 TCATATTCGTAAAGCAAAAACA TTTGAACCAATGACTCCCGC 27.3 50.0 52.7 58.8 1419-1978 bp 1.0 47.3
ndhC-trnM-CAU_p4 ATCATATTCGTAAAGCAAAAACA CCCGCCGTATGAAAGCAATA 26.1 50.0 53.3 58.4 1405-1964 bp 1.0 47.3
ndhC-trnM-CAU_p5 TCATATTCGTAAAGCAAAAACAT CCCGCCGTATGAAAGCAATA 26.1 50.0 53.3 58.4 1404-1963 bp 1.0 47.3