Pi
0.0534
MarkerSeek score
73.40
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | CGCGCTTGGTTTCTAAGTTC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1839-1866 bp | 0.666667 | 72.3 |
| trnT-UGU-trnL-UAA_p2 | CGCTTGGTTTCTAAGTTCGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1837-1864 bp | 0.666667 | 72.3 |
| trnT-UGU-trnL-UAA_p3 | GCGCTTGGTTTCTAAGTTCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1838-1865 bp | 0.666667 | 72.3 |
| trnT-UGU-trnL-UAA_p4 | GGCGAGCTTGAGGAATAGTC | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 58.1 | 58.1 | 1592-2089 bp | 0.666667 | 69.5 |
| trnT-UGU-trnL-UAA_p5 | GGCGAGCTTGAGGAATAGTC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.1 | 58.0 | 2081 bp | 0.333333 | 52.2 |