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psbZ-trnG-GCC

LSC · 41132-41693 · 562 bp

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Pi 0.0444

MarkerSeek score 83.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 36
pi: 0.0444
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.0	57.7	717-879 bp	1.0	84.6
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCG	ATACATAAATAGGGGGCGCG	55.0	50.0	58.0	57.6	761-923 bp	1.0	83.6
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	720-882 bp	1.0	83.1
psbZ-trnG-GCC_p4	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	713-875 bp	1.0	83.0
psbZ-trnG-GCC_p5	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.3	57.7	718-880 bp	1.0	82.7