Hotspot

atpH-atpI

LSC · 16155-17596 · 1442 bp

Back to Senegalia

Pi 0.0711

MarkerSeek score 77.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1442
variable_sites: 115
pi: 0.0711
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAAGAGCAGCAAAGCAAGTC	GAATATGGTCCGTGGGTTCC	50.0	55.0	58.0	58.0	1460-2231 bp	1.0	88.3
atpH-atpI_p2	AGCAAAGCAAGTCCAGGAAA	GAATATGGTCCGTGGGTTCC	45.0	55.0	57.9	58.0	1453-2224 bp	1.0	88.1
atpH-atpI_p3	CAAGAGCAGCAAAGCAAGTC	ACCATTCCTACTGACGGTCA	50.0	50.0	58.0	58.1	1541-2312 bp	1.0	87.9
atpH-atpI_p4	CAAGAGCAGCAAAGCAAGTC	GGCAAATAGGGGGTTTCCAA	50.0	50.0	58.0	58.1	1645-2416 bp	1.0	87.8
atpH-atpI_p5	CAAGAGCAGCAAAGCAAGTC	TTGGCAAATAGGGGGTTTCC	50.0	50.0	58.0	58.1	1647-2418 bp	1.0	87.8