Hotspot

atpF-atpH

LSC · 15222-15908 · 687 bp

Back to Senegalia

Pi 0.0451

MarkerSeek score 92.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 46
pi: 0.0451
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 92.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGGCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	749-813 bp	1.0	84.4
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.9	57.6	816-880 bp	1.0	84.0
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGGGG	50.0	55.0	57.9	58.6	814-878 bp	1.0	82.5
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCAGAGGGAAAAATACGGG	50.0	55.0	57.9	58.6	815-879 bp	1.0	82.5
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.9	57.3	819-883 bp	1.0	81.7