Hotspot

psbI-trnS-GCU

LSC · 9180-9570 · 391 bp

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Pi 0.0549

MarkerSeek score 75.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 28
pi: 0.0549
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TCTAACGATCCAGGACGTAA	TTCGAATCCCTCTCTTTCCG	45.0	50.0	55.4	57.0	381-455 bp	1.0	60.9
psbI-trnS-GCU_p2	TCTAACGATCCAGGACGTAA	GGGTTCGAATCCCTCTCTTT	45.0	50.0	55.4	56.9	384-458 bp	1.0	59.9
psbI-trnS-GCU_p3	TCTAACGATCCAGGACGTAA	GGGTTCGAATCCCTCTCTTTC	45.0	52.4	55.4	58.1	384-458 bp	1.0	59.9
psbI-trnS-GCU_p4	TCTAACGATCCAGGACGTAA	GGTTCGAATCCCTCTCTTTCC	45.0	52.4	55.4	58.1	383-457 bp	1.0	59.9
psbI-trnS-GCU_p5	ATCTAACGATCCAGGACGTA	TTCGAATCCCTCTCTTTCCG	45.0	50.0	55.2	57.0	382-456 bp	1.0	59.0