Hotspot

trnQ-UUG-psbK

LSC · 8030-8705 · 676 bp

Back to Senegalia

Pi 0.0719

MarkerSeek score 85.00

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 69
pi: 0.0719
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GATATGCTCTGGGACGGAAG	AGCAGCTTGCCAAACAAAAG	55.0	45.0	57.6	58.1	927-1006 bp	1.0	85.4
trnQ-UUG-psbK_p2	GATATGCTCTGGGACGGAAG	CTTACAGCAGCTTGCCAAAC	55.0	50.0	57.6	57.9	932-1011 bp	1.0	85.4
trnQ-UUG-psbK_p3	GACCAAAACCCGTTGCCTTA	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.7	58.1	885-964 bp	1.0	83.9
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.7	57.9	890-969 bp	1.0	83.9
trnQ-UUG-psbK_p5	GATATGCTCTGGGACGGAAG	GCACAAGCAAGGAAAACACA	55.0	45.0	57.6	57.7	1279-1359 bp	1.0	81.7