Hotspot

trnK-UUU-rps16

LSC · 4466-6005 · 1540 bp

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Pi 0.0668
MarkerSeek score 78.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1540
variable_sites
113
pi
0.0668
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG GGAACTTAATCCTAATCAACAAACGA 50.0 34.6 58.6 58.0 837-1667 bp 1.0 50.6
trnK-UUU-rps16_p2 GAGTCGCACTTAAAAGCCGA GGAACTTAATCCTAATCAACAAACGA 50.0 34.6 58.6 58.0 838-1668 bp 1.0 50.6
trnK-UUU-rps16_p3 AGTCGCACTTAAAAGCCGAG TCCTAATCAACAAACGAAATTCA 50.0 30.4 58.6 54.8 828-1658 bp 1.0 50.6
trnK-UUU-rps16_p4 GAGTCGCACTTAAAAGCCGA TCCTAATCAACAAACGAAATTCA 50.0 30.4 58.6 54.8 829-1659 bp 1.0 50.6
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG TGGAACTTAATCCTAATCAACAAACG 50.0 34.6 58.6 58.2 838-1668 bp 1.0 50.6