Hotspot

psbA-trnK-UUU

LSC · 1550-1838 · 289 bp

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Pi 0.0346

MarkerSeek score 72.10

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 289
variable_sites: 15
pi: 0.0346
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TCCAGTTACAAAAGCGACCC	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.1	58.6	392-404 bp	1.0	79.1
psbA-trnK-UUU_p2	TCCAGTTACAAAAGCGACCC	ACTAGTTCCGGGTTCGAGTC	50.0	55.0	58.1	58.8	382-394 bp	1.0	77.4
psbA-trnK-UUU_p3	TCCAGTTACAAAAGCGACCC	GACTAGTTCCGGGTTCGAGT	50.0	55.0	58.1	58.8	383-395 bp	1.0	77.4
psbA-trnK-UUU_p4	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	412-424 bp	1.0	77.1
psbA-trnK-UUU_p5	TCCAGTTACAAAAGCGACCC	ATCCGACTAGTTCCGGGTTC	50.0	55.0	58.1	58.9	387-399 bp	1.0	76.9