Hotspot

rbcL

LSC · 54581-56038 · 1458 bp

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Pi 0.00771

MarkerSeek score 49.30

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1458
variable_sites: 37
pi: 0.00771
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTCATTGAAA	50.0	45.0	57.1	58.0	1521 bp	1.0	74.5
rbcL_p2	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTCATTGAA	50.0	45.0	57.1	58.0	1522 bp	1.0	74.5
rbcL_p3	TCATGAGTTGTAGGGAGGGA	ATTGGGCCGAGTTTCATTGA	50.0	45.0	57.1	57.8	1523 bp	1.0	72.9
rbcL_p4	TTCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTCATTGAAA	47.6	45.0	57.8	58.0	1522 bp	1.0	72.4
rbcL_p5	TTCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTCATTGAA	47.6	45.0	57.8	58.0	1523 bp	1.0	72.4