Hotspot

ycf3-trnS-GGA

LSC · 43860-44770 · 911 bp

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Pi 0.0132

MarkerSeek score 67.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 46
pi: 0.0132
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.4	57.8	962-1046 bp	1.0	73.4
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.4	57.8	963-1047 bp	1.0	73.4
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1000-1084 bp	1.0	73.0
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1001-1085 bp	1.0	73.0
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	AGTTCCAATGCTACGCCTTC	45.0	50.0	56.4	58.3	964-1048 bp	1.0	72.8