Hotspot

psbC-trnS-UGA

LSC · 34816-35048 · 233 bp

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Pi 0.0209

MarkerSeek score 70.50

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 15
pi: 0.0209
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCTTCGTAGGGCATTTGTGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.9	58.0	422-430 bp	1.0	83.9
psbC-trnS-UGA_p2	TCTTCGTAGGGCATTTGTGG	GTCTTTCGGAGAGATGGCTG	50.0	55.0	57.9	58.1	430-438 bp	1.0	83.4
psbC-trnS-UGA_p3	TCTTCGTAGGGCATTTGTGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.9	58.2	363-371 bp	1.0	83.1
psbC-trnS-UGA_p4	TCTTCGTAGGGCATTTGTGG	TCTTTCGGAGAGATGGCTGA	50.0	50.0	57.9	58.1	429-437 bp	1.0	82.8
psbC-trnS-UGA_p5	CCGGTTCTTTCCATGACTCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.3	58.0	339-347 bp	0.916667	78.6