Hotspot

psbA

LSC · 312-1373 · 1062 bp

Back to Sempervivum

Pi 0.00113

MarkerSeek score 32.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	ACATAGGGAAAGCTGTGTGC	55.0	50.0	58.1	58.2	1602-1701 bp	1.0	83.1
psbA_p2	GATCCACTTGGCTACATCCG	CATAGGGAAAGCTGTGTGCA	55.0	50.0	58.1	58.2	1601-1700 bp	1.0	83.1
psbA_p3	CAATCCACTGCCTTGATCCA	ACATAGGGAAAGCTGTGTGC	50.0	50.0	57.9	58.2	1616-1715 bp	1.0	83.0
psbA_p4	CAATCCACTGCCTTGATCCA	CATAGGGAAAGCTGTGTGCA	50.0	50.0	57.9	58.2	1615-1714 bp	1.0	83.0
psbA_p5	AATCCACTGCCTTGATCCAC	ACATAGGGAAAGCTGTGTGC	50.0	50.0	57.9	58.2	1615-1714 bp	1.0	83.0