Hotspot

ndhF-rpl32

SSC · 110771-111370 · 600 bp

Back to Sempervivum

Pi 0.0129
MarkerSeek score 52.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
600
variable_sites
20
pi
0.0129
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAAGAAGAGGT TTAACGCTGCCCAATATCCC 50.0 50.0 56.5 58.0 738-746 bp 1.0 70.3
ndhF-rpl32_p2 CTGGAAGTGGAAGAAGAGGT AAAAGGCTTTTAACGCTGCC 50.0 45.0 56.5 57.9 747-755 bp 1.0 69.3
ndhF-rpl32_p3 CTGGAAGTGGAAGAAGAGGT GCTGCCCAATATCCCTTCTT 50.0 50.0 56.5 57.6 733-741 bp 1.0 67.6
ndhF-rpl32_p4 CTGGAAGTGGAAGAAGAGGT TAACGCTGCCCAATATCCCT 50.0 50.0 56.5 58.9 737-745 bp 1.0 63.6
ndhF-rpl32_p5 CTGGAAGTGGAAGAAGAGGT GCTTTTAACGCTGCCCAATA 50.0 45.0 56.5 57.1 742-750 bp 1.0 63.1