Hotspot

petL-petG

LSC · 64701-64872 · 172 bp

Back to Sempervivum

Pi 0.0182

MarkerSeek score 52.90

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 7
pi: 0.0182
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	ATTGGGCTGAGCAAGATACG	GTCCAACTGATCACCACGTT	50.0	50.0	58.0	58.1	309-321 bp	1.0	80.7
petL-petG_p2	ATTGGGCTGAGCAAGATACG	AAGTCCAACTGATCACCACG	50.0	50.0	58.0	57.8	311-323 bp	1.0	80.3
petL-petG_p3	GGCTGAGCAAGATACGACTT	GTCCAACTGATCACCACGTT	50.0	50.0	57.7	58.1	305-317 bp	1.0	78.7
petL-petG_p4	GCCTTTTTATTGGGCTGAGC	GTCCAACTGATCACCACGTT	50.0	50.0	57.7	58.1	317-329 bp	1.0	78.6
petL-petG_p5	GCCTTTTTATTGGGCTGAGC	AAGTCCAACTGATCACCACG	50.0	50.0	57.7	57.8	319-331 bp	1.0	78.2