Hotspot

petA-psbJ

LSC · 61943-62802 · 860 bp

Back to Sempervivum

Pi 0.0137

MarkerSeek score 56.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 25
pi: 0.0137
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTTATTTTGGCACA	CAGGATTGGGTTCATCCTTGT	42.9	47.6	57.4	57.9	961-968 bp	1.0	62.2
petA-psbJ_p2	GGCATCTGTTATTTTGGCACA	GGTACGGTAACTGGTATTCCG	42.9	52.4	57.4	57.9	1022-1029 bp	1.0	62.0
petA-psbJ_p3	GGCATCTGTTATTTTGGCACA	GTACGGTAACTGGTATTCCGG	42.9	52.4	57.4	57.9	1021-1028 bp	1.0	62.0
petA-psbJ_p4	TGGCATCTGTTATTTTGGCAC	GGTACGGTAACTGGTATTCCG	42.9	52.4	57.4	57.9	1023-1030 bp	1.0	62.0
petA-psbJ_p5	TGGCATCTGTTATTTTGGCAC	GTACGGTAACTGGTATTCCGG	42.9	52.4	57.4	57.9	1022-1029 bp	1.0	62.0