Hotspot

ycf4-cemA

LSC · 59452-60065 · 614 bp

Back to Sempervivum

Pi 0.0135

MarkerSeek score 64.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 614
variable_sites: 19
pi: 0.0135
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGGGGACATTCCATTGACTC	AAGAGAGATCAACCAGGGCA	55.0	50.0	57.9	58.3	791-803 bp	1.0	79.8
ycf4-cemA_p2	GGGGGACATTCCATTGACTC	AGAGAGATCAACCAGGGCAA	55.0	50.0	57.9	58.3	790-802 bp	1.0	79.8
ycf4-cemA_p3	TGACTCCACGCGAAATTGAA	AAGAGAGATCAACCAGGGCA	45.0	50.0	58.1	58.3	756-768 bp	1.0	79.4
ycf4-cemA_p4	TGACTCCACGCGAAATTGAA	AGAGAGATCAACCAGGGCAA	45.0	50.0	58.1	58.3	755-767 bp	1.0	79.4
ycf4-cemA_p5	TTGACTCCACGCGAAATTGA	AGAGAGATCAACCAGGGCAA	45.0	50.0	58.1	58.3	756-768 bp	1.0	79.4