Hotspot

trnL-UAA-trnF-GAA

LSC · 47438-47704 · 267 bp

Back to Sempervivum

Pi 0.0162

MarkerSeek score 52.50

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 10
pi: 0.0162
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TTCAAGTCCCTCTATCCCCA	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.0	58.0	306-310 bp	1.0	75.9
trnL-UAA-trnF-GAA_p2	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	342-346 bp	1.0	75.1
trnL-UAA-trnF-GAA_p3	TTCAAGTCCCTCTATCCCCA	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.0	58.1	357-361 bp	1.0	74.7
trnL-UAA-trnF-GAA_p4	TTCAAGTCCCTCTATCCCCA	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.0	57.9	318-322 bp	1.0	74.7
trnL-UAA-trnF-GAA_p5	ATCATGAGGGTTCAAGTCCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	56.9	58.0	316-320 bp	1.0	74.6