Hotspot

trnD-GUC-trnY-GUA

LSC · 30941-31248 · 308 bp

Back to Sempervivum

Pi 0.0119

MarkerSeek score 54.00

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 308
variable_sites: 9
pi: 0.0119
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTGGATACGTCGGGACTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.2	57.8	419-433 bp	1.0	81.9
trnD-GUC-trnY-GUA_p2	ATTGGATACGTCGGGACTGA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.2	57.8	418-432 bp	1.0	81.9
trnD-GUC-trnY-GUA_p3	ATTGGATACGTCGGGACTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.2	58.4	440-454 bp	1.0	80.7
trnD-GUC-trnY-GUA_p4	ATTGGATACGTCGGGACTGA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.2	57.6	439-453 bp	1.0	80.5
trnD-GUC-trnY-GUA_p5	ATTGGATACGTCGGGACTGA	CGGACTGTAAATTCGTTGGC	50.0	50.0	58.2	57.5	435-449 bp	1.0	79.4