Hotspot

psbM-trnD-GUC

LSC · 29876-30866 · 991 bp

Back to Sempervivum

Pi 0.0148

MarkerSeek score 66.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 991
variable_sites: 35
pi: 0.0148
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TCAATTGGTAAGAGCACCGC	45.0	50.0	55.8	58.6	1071-1107 bp	1.0	63.2
psbM-trnD-GUC_p2	AGGAACTAGAATGAACAGTGCA	TCAATTGGTAAGAGCACCGC	40.9	50.0	57.4	58.6	1078-1114 bp	1.0	60.0
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	AATTGGTAAGAGCACCGCC	45.0	52.6	55.8	58.1	1069-1105 bp	1.0	58.5
psbM-trnD-GUC_p4	GGAACTAGAATGAACAGTGCAG	TCAATTGGTAAGAGCACCGC	45.5	50.0	57.1	58.6	1077-1113 bp	1.0	57.5
psbM-trnD-GUC_p5	GGCAGTAGGAACTAGAATGAACA	TCAATTGGTAAGAGCACCGC	43.5	50.0	57.9	58.6	1084-1120 bp	1.0	56.2