Hotspot

trnG-GCC-trnR-UCU

LSC · 10168-10411 · 244 bp

Back to Sempervivum

Pi 0.0158

MarkerSeek score 59.40

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 244
variable_sites: 10
pi: 0.0158
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	TAGCCTTCCAAGCTACCGAT	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.2	55.6	308-331 bp	1.0	49.8
trnG-GCC-trnR-UCU_p2	TAGCCTTCCAAGCTACCGAT	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.2	54.6	302-325 bp	1.0	49.6
trnG-GCC-trnR-UCU_p3	TAGCCTTCCAAGCTACCGAT	ACCAAAGGTTTAGAAGACCTATGT	50.0	37.5	58.2	57.6	313-336 bp	1.0	49.2
trnG-GCC-trnR-UCU_p4	TGACGTCGACTATAACCCCT	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	57.6	55.6	327-350 bp	1.0	48.0
trnG-GCC-trnR-UCU_p5	TGACGTCGACTATAACCCCT	AGAAGACCTATGTCCTATCCA	50.0	42.9	57.6	54.6	321-344 bp	1.0	47.8