Hotspot

trnK-UUU-rps16

LSC · 4122-4801 · 680 bp

Back to Sempervivum

Pi 0.0167

MarkerSeek score 64.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 27
pi: 0.0167
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAGGCGGATGTTTTTAGGGA	50.0	45.0	58.2	57.1	745-771 bp	1.0	77.4
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	AGGCGGATGTTTTTAGGGAA	50.0	45.0	58.2	57.1	744-770 bp	1.0	77.4
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	AAGGCGGATGTTTTTAGGGA	50.0	45.0	58.2	57.1	744-770 bp	1.0	77.4
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	AGGCGGATGTTTTTAGGGAA	50.0	45.0	58.2	57.1	743-769 bp	1.0	77.4
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	GGCGGATGTTTTTAGGGAAC	50.0	50.0	58.2	57.1	742-768 bp	1.0	77.1