Hotspot

rps3

LSC · 87492-88148 · 657 bp

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Pi 0.00152

MarkerSeek score 59.60

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 1
pi: 0.00152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TCGTTGGATAGAACGTAAAGGA	ACACAAATATGCCGTGTCATG	40.9	42.9	56.9	57.2	735 bp	1.0	41.6
rps3_p2	TCGTTGGATAGAACGTAAAGGA	CACAAATATGCCGTGTCATGA	40.9	42.9	56.9	57.0	734 bp	1.0	40.1
rps3_p3	TCGTTGGATAGAACGTAAAGGA	AGTACACAAATATGCCGTGTCA	40.9	40.9	56.9	58.1	738 bp	1.0	40.1
rps3_p4	TCGTTGGATAGAACGTAAAGGAA	ACACAAATATGCCGTGTCATG	39.1	42.9	57.6	57.2	735 bp	1.0	40.1
rps3_p5	CGTTGGATAGAACGTAAAGGA	ACACAAATATGCCGTGTCATG	42.9	42.9	55.5	57.2	734 bp	1.0	40.1