Hotspot

rpl32-tRNA-UAG

SSC · 118728-119965 · 1238 bp

Back to Semiliquidambar

Pi 0.00162
MarkerSeek score 59.70
Primer available yes
Label rpl32-tRNA-UAG

Feature Metadata

label_name
rpl32-tRNA-UAG
feature_type
igs
region
SSC
length_bp
1238
variable_sites
2
pi
0.00162
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-tRNA-UAG_p1 GGGCAGCGTTAAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 1356-1364 bp 1.0 78.9
rpl32-tRNA-UAG_p2 GGGCAGCGTTAAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 1357-1365 bp 1.0 78.9
rpl32-tRNA-UAG_p3 GGGCAGCGTTAAAAGCTTTT TTTAGAAGATCCCATGCCGC 45.0 50.0 57.9 58.0 1421-1429 bp 1.0 78.7
rpl32-tRNA-UAG_p4 TAGGGCAGCGTTAAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1358-1366 bp 1.0 78.5
rpl32-tRNA-UAG_p5 TAGGGCAGCGTTAAAAGCTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1359-1367 bp 1.0 78.5