Hotspot

petA-psbJ

LSC · 67365-68431 · 1067 bp

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Pi 0.0169

MarkerSeek score 68.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1067
variable_sites: 18
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	GCCGATACTACTGGAAGGAT	45.0	50.0	58.0	56.2	1297-1299 bp	1.0	66.5
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	GCCGATACTACTGGAAGGAT	50.0	50.0	57.8	56.2	1287-1289 bp	1.0	65.7
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	GCCGATACTACTGGAAGGATT	45.0	47.6	58.0	57.0	1297-1299 bp	1.0	64.6
petA-psbJ_p4	ACGTGTACAAGGCCTTTTGT	GCCGATACTACTGGAAGGATTC	45.0	50.0	58.0	58.1	1297-1299 bp	1.0	64.2
petA-psbJ_p5	TCCATTACGTGTACAAGGCC	GCCGATACTACTGGAAGGAT	50.0	50.0	57.6	56.2	1303-1305 bp	1.0	63.8