Hotspot

rps4-tRNA-UGU

LSC · 49441-49775 · 335 bp

Back to Semiliquidambar

Pi 0.00597
MarkerSeek score 56.70
Primer available yes
Label rps4-tRNA-UGU

Feature Metadata

label_name
rps4-tRNA-UGU
feature_type
igs
region
LSC
length_bp
335
variable_sites
2
pi
0.00597
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-tRNA-UGU_p1 TAGTGAGTCCCGGTAAAGCT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.8 57.9 465 bp 1.0 77.5
rps4-tRNA-UGU_p2 CTCCCAGACGGCGTATTTTT CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.3 57.9 447 bp 1.0 77.2
rps4-tRNA-UGU_p3 GGAACGCGATTGGTGTCTAA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.3 57.9 512 bp 1.0 77.0
rps4-tRNA-UGU_p4 AGATCACTTCCGGCTCTAGG CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.3 57.9 493 bp 1.0 76.9
rps4-tRNA-UGU_p5 TAGTGAGTCCCGGTAAAGCT GGTTCGATTCCGATAGCTGG 50.0 55.0 57.8 58.2 424 bp 1.0 76.8