Hotspot

psaA-ycf3

LSC · 44980-45649 · 670 bp

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Pi 0.00149

MarkerSeek score 59.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 1
pi: 0.00149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCGGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	867 bp	1.0	79.0
psaA-ycf3_p2	TGAGAAATGACCGGGTTTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	869 bp	1.0	78.2
psaA-ycf3_p3	TGAGAAATGACCGGGTTTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	796 bp	1.0	78.0
psaA-ycf3_p4	TGAGAAATGACCGGGTTTGG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.1	58.2	800 bp	1.0	77.8
psaA-ycf3_p5	TGAGAAATGACCGGGTTTGG	CGGAGGTTTGGTTCGATCAA	50.0	50.0	58.1	58.2	882 bp	1.0	77.8