Hotspot

tRNA-GCA-petN

LSC · 29630-30408 · 779 bp

Back to Semiliquidambar

Pi 0.00257

MarkerSeek score 60.20

Primer available yes

Label tRNA-GCA-petN

Feature Metadata

label_name: tRNA-GCA-petN
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 2
pi: 0.00257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	901 bp	1.0	78.5
tRNA-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	845 bp	1.0	78.3
tRNA-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	962 bp	1.0	76.9
tRNA-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	906 bp	1.0	76.7
tRNA-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	863 bp	1.0	76.1