Hotspot

tRNA-UUU-rps16

LSC · 4365-5314 · 950 bp

Back to Semiliquidambar

Pi 0.00106
MarkerSeek score 59.80
Primer available yes
Label tRNA-UUU-rps16

Feature Metadata

label_name
tRNA-UUU-rps16
feature_type
igs
region
LSC
length_bp
950
variable_sites
1
pi
0.00106
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-UUU-rps16_p1 CCGATGGTATGGACGAATCC GGTGCTCAACCTACAGGAAC 55.0 55.0 57.9 58.2 1129-1131 bp 1.0 78.2
tRNA-UUU-rps16_p2 CCGATGGTATGGACGAATCC AGAAGGCGGAGGTTTTTACG 55.0 50.0 57.9 58.2 1092-1094 bp 1.0 78.2
tRNA-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1068-1070 bp 1.0 77.5
tRNA-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1067-1069 bp 1.0 77.5
tRNA-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGAAGGCGGAGGTTTTTACG 50.0 50.0 58.2 58.2 1030-1032 bp 1.0 77.5