Hotspot

matK-tRNA-UUU

LSC · 3609-4327 · 719 bp

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Pi 0.00139

MarkerSeek score 59.50

Primer available yes

Label matK-tRNA-UUU

Feature Metadata

label_name: matK-tRNA-UUU
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 1
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-tRNA-UUU_p1	GGAAGTCATGTTGCCGAGAT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	798-799 bp	1.0	78.5
matK-tRNA-UUU_p2	GGAAGTCATGTTGCCGAGAT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	797-798 bp	1.0	78.5
matK-tRNA-UUU_p3	GGAAGTCATGTTGCCGAGAT	CGTCGTGCAATCCAATCTCT	50.0	50.0	58.0	58.4	888-889 bp	1.0	77.0
matK-tRNA-UUU_p4	GGAAGTCATGTTGCCGAGAT	GGGTTGCTAACTCAACGGTA	50.0	50.0	58.0	57.5	811-812 bp	1.0	76.2
matK-tRNA-UUU_p5	GGAAGTCATGTTGCCGAGAT	TTCGGGTTGCTAACTCAACG	50.0	50.0	58.0	58.5	814-815 bp	1.0	75.9