Hotspot

ycf1

SSC · 130261-135609 · 5349 bp

Back to Semiaquilegia

Pi 0.0109

MarkerSeek score 47.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5349
variable_sites: 58
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTCGTATGCACACGTGAAA	TGTCTACTGCTTCGATTGAGA	45.0	42.9	57.9	56.5	219 bp	1.0	61.1
ycf1_p2	GCTTACTCGTATGCACACGT	TGTCTACTGCTTCGATTGAGA	50.0	42.9	58.4	56.5	223 bp	1.0	59.2
ycf1_p3	TGCTTACTCGTATGCACACG	TGTCTACTGCTTCGATTGAGA	50.0	42.9	58.4	56.5	224 bp	1.0	59.2
ycf1_p4	ACTCGTATGCACACGTGAAA	TTGTCTACTGCTTCGATTGAGA	45.0	40.9	57.9	57.2	220 bp	1.0	58.8
ycf1_p5	GCTTACTCGTATGCACACGT	TTGTCTACTGCTTCGATTGAGA	50.0	40.9	58.4	57.2	224 bp	1.0	56.9